A simple method for detection of a novel coronavirus (SARS-CoV-2) using one-step RT-PCR followed by restriction fragment length polymorphism
Detection Principle
NucleicAcid-PCR based
Target
RdRP, E-gene
Testing Method Category
RT-PCR
Testing Method
in house RT-PCR + RFLP
Testing Method - Additional Info
assays were designed to target conserved regions of the RdRP gene and E gene
Reported Performance
sensitivity: 95%; specificity: 100%
Sample Size
50 samples
Assay Type
Peer-reviewed
yes
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