Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits
Detection Principle
NucleicAcid-PCR based
Testing Method Category
RT-PCR
Testing Method - Additional Info
Three different methods for the extraction nucleic acid The first method involves incubating the specimen with proteinase K, and then heat treatment at 98?°C for 5?min (PKH); the second method involves heat treatment at 98?°C for 5?min without proteinase K pre-incubation (heat only); the third method involves no pre-processing steps (direct
Reported Performance
PKH had significantly higher positive rate in SARS-CoV-2 RT-PCR (80 %) than those of heat only (58 %; P?=?0.001) or direct (56 %; P?=?0.002). The median Ct value was significantly earlier for PKH (median Ct: 37.0, IQR 31.7–40) than that of heat only (median Ct: 40, IQR 36.2–41; P?<?0.0001) and direct (median Ct, 37.5; IQR 33.9–41.0; P?=?0.0049).
Sample Size
50 specimens, including 25 nasopharyngeal swab (NPS) and 25 posterior oropharyngeal saliva specimens
Peer-reviewed
yes
The database contains available information from scientific literature that is being updated periodically. Please note that the provided information (as retrieved from analysed papers) is provided only for devices commercially available with CE-IVD mark. Acknowledgements