Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?
Detection Principle
Mixed
Target
N gene (RT-PCR); IgG (ELISA)
Testing Method Category
Mixed
Testing Method
kits
Testing Method - Additional Info
effect of 3 heat inactivation protocols on qRT-PCR EXPRESS One-Step Superscript, ThermoFisher Scientific, Waltham, Massachusetts & anti-SARS-CoV-2 ELISA for immunoglobulin class G (EI 2606-9601 G, Euroimmun AG, Lübeck, Germany)
Reported Performance
remark: Regardless of the inactivation protocol and the type of samples, a 4 Log10 TCID50 reduction was observed. The protocols 56 °C-30 min and 60 °C-60 min had little influence on the RNA copies detection, whereas 92 °C-15 min drastically reduced the limit of detection. 56 °C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test
Sample Size
spiked samples
Peer-reviewed
yes
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