Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Detection Principle
NucleicAcid-PCR based
Target
Nsp3, Spike, ORF8, N gene
Testing Method Category
LAMP
Testing Method
LAMP
Testing Method - Additional Info
in house RT-LAMP
Reported Performance
LOD: 100 copies RNA; remark: Cross-reactivity of RT-LAMP assays to other human coronaviruses was not observed
Sample Size
dilution of standard and viral RNA
Peer-reviewed
yes
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