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COVID-19 In Vitro Diagnostic Devices and Test Methods Database

Scientific literature on COVID-19 Test Methods and Devices - detail

SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

Detection Principle
NucleicAcid-PCR based
E, S, RdRP
Testing Method Category
Testing Method
Testing Method - Additional Info
comparison between direct PCR using SeeGene AllplexTM 2019-nCoV rRT-PCR with RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics)
Reported Performance
Reamrk: i) SeeGene and Altona kits provide similar efficiency, ii) direct rRT-PCR without RNA extraction is possible if samples are collected in UTM or molecular water; ii), specimens stored in water should be screened rapidly, iii) RNA extraction is necessary if samples are in saline water or Hanks medium.
Sample Size
95 samples

The database contains available information from scientific literature that is being updated periodically. Please note that the provided information (as retrieved from analysed papers) is provided only for devices commercially available with CE-IVD mark. Acknowledgements