Commercial stocks of SARS-CoV-2 RNA may report low concentration values, leading to artificially increased apparent sensitivity of diagnostic assays
Detection Principle
NucleicAcid-PCR based
Target
/
Testing Method Category
PCR_KIT
Testing Method
kit and RT-dPCR
Testing Method - Additional Info
CDC RT-PCR and dPCR test on commercial nucleic acid stocks (synthetic RNA, plasmid DNA and genomic RNA)
Reported Performance
Remark: identification of discrepancies in the analytical sensitivity (LOD) of the CDC?recommended RT?qPCR protocol. RT?dPCR revealed there are likely errors in the supplier?reported concentrations of the nucleic acid stocks.
Sample Size
/
Peer-reviewed
no
The database contains available information from scientific literature that is being updated periodically. Please note that the provided information (as retrieved from analysed papers) is provided only for devices commercially available with CE-IVD mark. Acknowledgements