Overcoming Supply Shortage for SARS-CoV-2 Detection by RT-qPCR.
Testing Method Category
Testing Method - Additional Info
compare results with saliva instead of nasopharingeal swabs, lower primer/probe concentration and thermocycling time, 0.45% saline instead of guanidine hydrochloride transport media
LOD: 7.3 cp/reaction (regular RT-qPCR), 23.7 cp/reaction (primer/probe lower concentration),44 cp/reaction (lower thermocycling time) ; remarks: saliva is not an appropriate substitute for nasopharyngeal swabs, saline and guanidine hydrochloride have similar diagnostic capabilities and detection is possible after 10 days storage in both media (for these Ct values are compared)
The database contains available information from scientific literature that is being updated periodically. Please note that the provided information (as retrieved from analysed papers) is provided only for devices commercially available with CE-IVD mark. Acknowledgements