Capillary Electrophoresis of PCR fragments with labelled primers for testing the SARS-Cov-2
Detection Principle
NucleicAcid-PCR based
Target
2 fragments of the SARS-CoV-2
Testing Method Category
Other sequencing method(s)
Testing Method
in house method
Testing Method - Additional Info
The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. One of the primers for each pair was 5’-end fluorochrome labelled. The amplifications were subjected to capillary electrophoresis in ABI3130 sequencers to visualize the fluorescent peaks. Alternative method to identify the SARS-Cov-2 genome that could be scaled to the analysis of approximately 100 samples in less than 5 hours. By combining a standard PCR with capillary electrophoresis our approach would overcome the limits imposed to many labs by the qtPCR (lack of reactive and real-time PCR equipment) and increase the testing capacity.
Reported Performance
Remark: Two SARS-Cov-2 fragments successfully amplified in the positive samples, while no fluorescent peaks in the negative samples. Screening of 96 samples in less than 5 hours.
Sample Size
naso-pharingeal RNA from 20 positive and 10 negative samples
Peer-reviewed
no
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