Rapid detection of SARS-CoV-2 by low volume real-time single tube reverse transcription recombinase polymerase amplification using an exo probe with an internally linked quencher (exo-IQ)
Detection Principle
NucleicAcid-Others
Target
N gene
Testing Method Category
RT_PCR
Testing Method
in house reverse transcription recombinase polymerase amplification
Testing Method - Additional Info
Generation of a suitable set of reverse transcription recombinase polymerase amplification (RT-RPA) primer and exonuclease probe, internally quenched (exo-IQ) probe sequences targeting the SARS-CoV-2 N gene
Reported Performance
Sensitivity 100%, specificity: 100%
Sample Size
19 samples
Peer-reviewed
yes
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